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PRIOR FUNDING
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"Development of new diagnostic assays and identification of novel
therapeutic targets for Ewing’s Sarcoma using single molecule RNA
imaging"
Principal Investigator: Dr. Mona Batish
Institution: Rutgers - New Jersey Medical School

Ewing's sarcoma (ES) is a prevalent pediatric, bone and soft tissue tumor that
is caused by a chromosomal translocation between Ewing's sarcoma breakpoint
region 1 (EWSR1) and Friend leukemia integration 1 (FLI1). This translocation
leads to the formation of the fused EWS/FLI1 gene, which codes for an
aberrant transcription factor, EWS/FLI1 the underlying cause for ES. We are
utilizing the technique of single molecule fluorescence in situ hybridization
(smFISH) to study the interactions of EWS/FLI1 to understand the mechanism
of ES progression. This will enable identification of novel therapeutic targets
and biomarkers for diagnosis. We have also adapted smFISH, to develop a new
diagnostic method called Fusion-FISH. We plan to couple Fusion-FISH with
automated image-analysis computer programs, to identify the location of
affected cells among the normal cells in a tissue sample, and to count the
number of gene fusion transcripts. The overall goal of the project is to develop
sensitive diagnostic assays for preventive screening and for therapy follow up
while expanding our understanding of underlying molecular mechanisms of
cancer initiation and progression, thereby enabling development of more
effective therapies.
The above project description is supplied by the Principal Investigator